This manual describes how to use bd facsdiva software. Running a basic 2 color flow cytometry experiment in bd. Bd facsdiva software features bd facsdiva software. Bd facsdiva software v6 with sample preppers bd facsdiva software v6 with spa ii and lwa sample preppers bd facsdiva software v6 with spa iii and lwa sample. A practical approach to multicolor flow cytometry for immunophenotyping. This video describes how to set up an experiment in facs diva version 8.
Cytometry win upgrades on diva computers edited subject. Exports fcs files that can be imported into diva, flowjo or other flow analysis software. If you use a becton dickinson bd cytometer in your lab, the chances are you are acquiring your data using diva software. You can change gates and plots, add statistics and create pdf files. The functionality and stability of the software combined with excellent and timely support has made it an important tool for research cytometry at tsri. Fcs express supports flow cytometry index sorting data files from all major platforms including influx bd biosciences, summit software beckman coulter, diva. Standardizing application setup across multiple flow. The bd facsdiva software reference manual assumes you have a working knowledge of basic microsoft windows operation. Press question mark to learn the rest of the keyboard shortcuts. Cyflogic a free flow cytometry data analysis software. Mar 01, 2015 index sorting from facsdiva to flowjo we recently upgraded our facsaria to facsdiva 8 running on windows 7 primarily for the ability to do index sorting. We work closely with other core facilities to provide comprehensive and integrative services to solve the imaging and cytometry challenges presented by cuttingedge research programs. The bd facsdiva installer installs the following applications. Bd biosciences brand of digital flow cytometers, including the facscanto, the lsrii, facsaria and fortessa, utilize a software acquisition program known as facsdiva.
Training faculty of medicine flow cytometry facility. The facility provides a wide variety of cell analysis and sorting services. Bd facsdiva operating software is common across many bd cell analyzers and cell sorters, including bd lsr and bd facsaria systems, giving researchers application flexibility. How to analyze facs data and prepare flow cytometry figures for scientific papers written by tim bushnell, phd it would be possible to describe everything scientifically, but it would make no sense. Flowjo is your biggest fan and strives to be an outstanding source of support. Bd biosciences provides flow cytometers, reagents, tools and a wide range of services to support the work of researchers and clinicians who understand disease and improve care.
Adjusting the diva config file seems to work more consistently. With the release and use of the becton dickenson facs diva software, the use of area as the default parameter came into play. Hallway workstation for diva exporting diva away from the lsriis, a different system is required. Refer to the information supplied by the manufacturer for applicationspecific limitations. This catalog strives to be comprehensive to include as much free flow cytometry software as possible. Fcs express supports flow cytometry index sorting data files from all major platforms including influx bd biosciences, summit software beckman coulter, diva bd biosciences, and chorus bd biosciences. Our instrument laboratory provides training and consultation services for researchers whose work involves flow cytometry, confocal microscopy and geldocumentation. The importance of area scaling with facs diva software. Intracellular flow multicolor flow cytometry stem cell research tcell research support support sales and support faq technical support training resources and tools. It has all regular analysis capabilities, such as dot plot, histogram and statistics.
Diva software on the dedicated hallway workstation computers can open only one of the lsriis databases and will present all of the unprocessed data just as it would appear at the original cytometer computer. However, diva has come along way over the past 10 years and many improvements have been made to help enduser. Ive had bad luck fixing this in the environmental variables diva sometimes refuses to open. Bd facsdiva software provides one of the most complete and robust feature sets. Accelerate your discovery with the leading platform for singlecell flow cytometry analysis. Diva is aptly named as it can be a difficult program to master. These pages offer you a newest version of cyflogic, help. If suitable markers are available to separate the cells being analysed from the other events, as few as 1 cell in 10 7 can be measured. Can anybody help me with how to measure mfi median fluorescence intensity using bd facsdiva software in flow cytometry. Tubes are added underneath the specimen icon in an experiment.
A large gap between initial training and followup sessions is not recommended. In order to achieve a count of the desired statistical significance, only the total number of positive events n is relevant. Index sorting from facsdiva to flowjo flow cytometry news. We recommend using these resources to aid in the design, execution, and publication of your studies. Can anybody help me with how to measure mfi median.
The field of flow cytometry is constantly evolving. Getting used to a brand new set of diva issues and quirks has been difficult, but we soldiered on nonetheless. Since 1979, the flow cytometry facility has provided university of iowa investigators with stateoftheart, laserbased instrumentation for basic cell research. Does anyone know if the cba cytokine assay offered by bdbiosciences is compatible to run with bdfacsdiva software on a flow cytometer vs the bdfacssuite. Has anyone got a way to improve the speed of the facs diva software. Software training is quite intensive and it will take repeated solo practice sessions to become proficient. To develop new innovations and ideas for the analysis. Running a basic 2 color flow cytometry experiment in bd facs diva duration. Diva will create a statistics box based on the dotplots or histograms. Bd facsdivatm software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow. I have recently developed cloud based flow cytometry.
History revision date change made 337647 rev a 0104 initial release for bd facsdiva software version 4. Flow cytometry and cell sorting nc state veterinary. Bd facsdiva software reference manual for version 8. To provide free, user friendly and effective analysis tool for flow cytometry community. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents. For information on how to operate and maintain your flow cytometer, refer to your cytometer manual. Since the initial commercialization of flow cytometry fc and fluorescence activated cell sorting facs in 1968, they have undergone significant improvements. Bd facsdiva software efficiently controls the setup, acquisition, and analysis of flow cytometry data from the bd facscanto ii workstation. Bd facsdiva software is for in vitro diagnostic ivd use when used with ivd reagents and cytometers. Diva software is used to acquire your cytometry data on lsrii, lsrfortessa, cantoii and aria cell sorters. Bd facsdiva bead lot files resources software bd biosciences. Standardizing application setup across multiple flow cytometers using bd facsdiva version 6 software bd biosciences march 2012 page 5 compiling data needed to standardize application settings in a laboratory that has multiple sites andor cytometers, a cytometer baseline report needs to be generated for each cytometer. Bd facsdiva software provides one of the most complete and robust feature sets available for flow cytometry. These are your samples that will be run on the flow cytometer.
The mission of the flow cytometry core at penn state college of medicine is to facilitate cuttingedge research by providing stateoftheart fluorescenceactivated cell sorting and analytical services at reasonable hourly rates. Bd biosciences facscanto ii flow cytometer features. May 19, 2015 if you use a becton dickinson bd cytometer in your lab, the chances are you are acquiring your data using diva software. Setting instrument voltages faculty of medicine flow. We have also added some demonstrations of commercial packages. Feb 01, 2018 3 flow cytometry core, houston methodist research institute, 6670 bertner st r7219 houston, tx 77030, usa. Bd facsdiva software features bd facsdiva software bd. Flow cytometry is well suited to the detection of rare events. As such, the use of area as a calculated parameter, methods were needed to be employed to ensure doublet discrimination and proper display on standard fscssc. Can anyone recommend a good free facs analysing software. The bd facsdiva software reference manual assumes that you have a working. How to analyze facs data and prepare flow cytometry figures.
It will take approximately 1012 hours to solidify the concepts learned during the course. In addition, cyflogic offers new innovative tools for your data analysis. Catalog of free flow cytometry software purdue university. Flow cytometry and cell sorting penn state college of. A pdf version of this page is available here advanced instrument.
Can analyze up to nine colors and utilizes bd diva software for acquisition. A practical guide for using flow cytometry and cell sorting, including extensive discussion on hardware, suppliers, reagents, and software. Ryan duggan flow cytometry facility university of chicago on aug 9, 2005, at 11. Cyflogic is a flow cytometry data analysis tool for microsoft windows enviroment. Expanding confidence and flexibility in todays integrated laboratory bd facsdiva software is a collection of rich tools for flow cytometer and application setup, data acquisition, and data analysis that help streamline flow cytometry workflows for todays busy laboratory.
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